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CAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.

ABSTRACT: In mammals, transcriptional autorepression by Period (PER) and Cryptochrome (CRY) protein complexes is essential for the generation of circadian rhythms. We have identified CAVIN-3 as a new, cytoplasmic PER2-interacting protein influencing circadian clock properties. Thus, CAVIN-3 loss- and gain-of-function shortened and lengthened, respectively, the circadian period in fibroblasts and affected PER:CRY protein abundance and interaction. While depletion of protein kinase C? (PKC?), a known partner of CAVIN-3, had little effect on circadian gene expression, CAVIN-3 required the PKC?-binding site to exert its effect on period length. This suggests the involvement of yet uncharacterized protein kinases. Finally, CAVIN-3 activity in circadian gene expression was independent of caveolae.

SUBMITTER: Schneider K

PROVIDER: S-EPMC3512403 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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CAVIN-3 regulates circadian period length and PER:CRY protein abundance and interactions.

Schneider Kim K Köcher Thomas T Andersin Teemu T Kurzchalia Teymuras T Schibler Ueli U Gatfield David D

EMBO reports 20121019 12

In mammals, transcriptional autorepression by Period (PER) and Cryptochrome (CRY) protein complexes is essential for the generation of circadian rhythms. We have identified CAVIN-3 as a new, cytoplasmic PER2-interacting protein influencing circadian clock properties. Thus, CAVIN-3 loss- and gain-of-function shortened and lengthened, respectively, the circadian period in fibroblasts and affected PER:CRY protein abundance and interaction. While depletion of protein kinase Cδ (PKCδ), a known partne ...[more]

PMID: 23079727

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