Synthesis and application of an N-acylated l-homoserine lactone derivatized affinity matrix for the isolation of quorum sensing signal receptors.
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ABSTRACT:
SUBMITTER: Praneenararat T
PROVIDER: S-EPMC3513337 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Publications
Synthesis and application of an N-acylated l-homoserine lactone derivatized affinity matrix for the isolation of quorum sensing signal receptors.
Praneenararat Thanit T Beary Teresa M J TM Breitbach Anthony S AS Blackwell Helen E HE
Bioorganic & medicinal chemistry letters 20110422 17
The design and synthesis of an agarose resin functionalized with a Gram-negative quorum sensing (QS) signaling molecule analogue is described. The modified resin was utilized in affinity pull-down assays to successfully isolate QscR, a LuxR-type QS receptor from Pseudomonas aeruginosa. This resin may facilitate the identification of novel QS signal receptors using affinity chromatography techniques. ...[more]
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