Dataset Information

A GAL4-driver line resource for Drosophila neurobiology.

ABSTRACT: We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.

SUBMITTER: Jenett A

PROVIDER: S-EPMC3515021 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A GAL4-driver line resource for Drosophila neurobiology.

Jenett Arnim A Rubin Gerald M GM Ngo Teri-T B TT Shepherd David D Murphy Christine C Dionne Heather H Pfeiffer Barret D BD Cavallaro Amanda A Hall Donald D Jeter Jennifer J Iyer Nirmala N Fetter Dona D Hausenfluck Joanna H JH Peng Hanchuan H Trautman Eric T ET Svirskas Robert R RR Myers Eugene W EW Iwinski Zbigniew R ZR Aso Yoshinori Y DePasquale Gina M GM Enos Adrianne A Hulamm Phuson P Lam Shing Chun Benny SC Li Hsing-Hsi HH Laverty Todd R TR Long Fuhui F Qu Lei L Murphy Sean D SD Rokicki Konrad K Safford Todd T Shaw Kshiti K Simpson Julie H JH Sowell Allison A Tae Susana S Yu Yang Y Zugates Christopher T CT

Cell reports 20121011 4

We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patt ...[more]

PMID: 23063364

Similar Datasets

Project description:The ability to express a gene of interest in a spatio-temporal manner using Gal4-UAS system has allowed the use of Drosophila model to study various biological phenomenon. During Drosophila eye development, a synchronous wave of differentiation called Morphogenetic furrow (MF) initiates at the posterior margin resulting in differentiation of retinal neurons. This synchronous differentiation is also observed in the differentiating retina of vertebrates. Since MF is highly dynamic, it can serve as an excellent model to study patterning and differentiation. However, there are not any Gal4 drivers available to observe the gain- of- function or loss- of- function of a gene specifically along the dynamic MF. The decapentaplegic (dpp) gene encodes a secreted protein of the transforming growth factor-beta (TGF-beta) superfamily that expresses at the posterior margin and then moves with the MF. However, unlike the MF associated pattern of dpp gene expression, the targeted dpp-Gal4 driver expression is restricted to the posterior margin of the developing eye disc. We screened GMR lines harboring regulatory regions of dpp fused with Gal4 coding region to identify MF specific enhancer of dpp using a GFP reporter gene. We employed immuno-histochemical approaches to detect gene expression. The rationale was that GFP reporter expression will correspond to the dpp expression domain in the developing eye. We identified two new dpp-Gal4 lines, viz., GMR17E04-Gal4 and GMR18D08-Gal4 that carry sequences from first intron region of dpp gene. GMR17E04-Gal4 drives expression along the MF during development and later in the entire pupal retina whereas GMR18D08-Gal4 drives expression of GFP transgene in the entire developing eye disc, which later drives expression only in the ventral half of the pupal retina. Thus, GMR18D08-Gal4 will serve as a new reagent for targeting gene expression in the ventral half of the pupal retina. We compared misexpression phenotypes of Wg, a negative regulator of eye development, using GMR17E04-Gal4, GMR18D08-Gal4 with existing dpp-Gal4 driver. The eye phenotypes generated by using our newly identified MF specific driver are not similar to the ones generated by existing dpp-Gal4 driver. It suggests that misexpression studies along MF needs revisiting using the new Gal4 drivers generated in our studies.

Dataset Information

A GAL4-driver line resource for Drosophila neurobiology.

Publications

A GAL4-driver line resource for Drosophila neurobiology.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets