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G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.

ABSTRACT: We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.

SUBMITTER: Evans CJ

PROVIDER: S-EPMC2754220 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila.

Evans Cory J CJ Olson John M JM Ngo Kathy T KT Kim Eunha E Lee Noemi E NE Kuoy Edward E Patananan Alexander N AN Sitz Daniel D Tran Phuongthao P Do Minh-Tu MT Yackle Kevin K Cespedes Albert A Hartenstein Volker V Call Gerald B GB Banerjee Utpal U

Nature methods 20090726 8

We combined Gal4-UAS and the FLP recombinase-FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale. ...[more]

PMID: 19633663

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