Ontology highlight
ABSTRACT:
SUBMITTER: Ferreira-Cerca S
PROVIDER: S-EPMC3515705 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Ferreira-Cerca Sébastien S Sagar Vatsala V Schäfer Thorsten T Diop Momar M Wesseling Anne-Maria AM Lu Haiyun H Chai Eileen E Hurt Ed E LaRonde-LeBlanc Nicole N
Nature structural & molecular biology 20121028 12
Ribosome synthesis involves dynamic association of ribosome-biogenesis factors with evolving preribosomal particles. Rio2 is an atypical protein kinase required for pre-40S subunit maturation. We report the crystal structure of eukaryotic Rio2-ATP-Mg(2+) complex. The active site contains ADP-Mg(2+) and a phosphoaspartate intermediate typically found in Na(+), K(+) and Ca(2+) ATPases but not protein kinases. Consistent with this finding, ctRio2 exhibits a robust ATPase activity in vitro. In vivo, ...[more]