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Biomimetic assembly of proteins into microcapsules on oil-in-water droplets with structural reinforcement via biomolecular-recognition-based cross-linking of surface peptides.

ABSTRACT: By mimicking the stabilization of bacterial membranes with S-layer proteins, a novel process to fabricate highly stable protein microcapsules is introduced. In this strategy, engineered collagen peptides with site-specific biotinylation are assembled into microcapsules on the oil-in-water droplets, and the resulting microcapsules are reinforced by biomolecular-recognition-based cross-linking with the protein. Furthermore the microcapsules are shown to be versatile scaffolds for developing functionalized hierarchical colloidosomes for important biotechnological applications.

SUBMITTER: Maeda Y

PROVIDER: S-EPMC3516996 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Biomimetic assembly of proteins into microcapsules on oil-in-water droplets with structural reinforcement via biomolecular-recognition-based cross-linking of surface peptides.

Maeda Yoshiaki Y Wei Zengyan Z Matsui Hiroshi H

Small (Weinheim an der Bergstrasse, Germany) 20120229 9

By mimicking the stabilization of bacterial membranes with S-layer proteins, a novel process to fabricate highly stable protein microcapsules is introduced. In this strategy, engineered collagen peptides with site-specific biotinylation are assembled into microcapsules on the oil-in-water droplets, and the resulting microcapsules are reinforced by biomolecular-recognition-based cross-linking with the protein. Furthermore the microcapsules are shown to be versatile scaffolds for developing functi ...[more]

PMID: 22378709

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