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The ?-ketoglutarate/Fe(II)-dependent dioxygenase VldW is responsible for the formation of validamycin B.

ABSTRACT: From A to B: Through detailed biochemical investigations, we discovered that VldW, an ?-ketoglutarate/Fe(II)-dependent dioxygenase, regioselectively hydroxylates validamycin A to validamycin B. The results provide insights into the biosynthesis of hydroxylated validamycins and could be used to control the metabolic outcomes of the validamycin pathway.

SUBMITTER: Almabruk KH 

PROVIDER: S-EPMC3517013 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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The α-ketoglutarate/Fe(II)-dependent dioxygenase VldW is responsible for the formation of validamycin B.

Almabruk Khaled H KH   Asamizu Shumpei S   Chang Ada A   Varghese Sheril G SG   Mahmud Taifo T  

Chembiochem : a European journal of chemical biology 20120907 15

From A to B: Through detailed biochemical investigations, we discovered that VldW, an α-ketoglutarate/Fe(II)-dependent dioxygenase, regioselectively hydroxylates validamycin A to validamycin B. The results provide insights into the biosynthesis of hydroxylated validamycins and could be used to control the metabolic outcomes of the validamycin pathway. ...[more]

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