Ontology highlight
ABSTRACT:
SUBMITTER: van de Bospoort R
PROVIDER: S-EPMC3518216 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
van de Bospoort Rhea R Farina Margherita M Schmitz Sabine K SK de Jong Arthur A de Wit Heidi H Verhage Matthijs M Toonen Ruud F RF
The Journal of cell biology 20121201 6
Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely unknown. We quantified activity-dependent DCV release in hippocampal neurons at single vesicle resolution. DCVs fused preferentially at synaptic terminals. DCVs also fused at extrasynaptic sites but only after prolonged stimulation. In munc13-1/2-null mutant neurons, synaptic DCV release was reduced but not abolished, and synaptic prefere ...[more]