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Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons.

ABSTRACT: Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechanisms and is easily adapted to investigate fusion principles of other secretory organelles. For complete details on the use and execution of this protocol, please refer to Persoon et al. (2019).

SUBMITTER: Moro A

PROVIDER: S-EPMC7890040 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons.

Moro Alessandro A Hoogstraaten Rein I RI Persoon Claudia M CM Verhage Matthijs M Toonen Ruud F RF

STAR protocols 20210203 1

Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechan ...[more]

PMID: 33659902

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