Ontology highlight
ABSTRACT:
SUBMITTER: Poole CB
PROVIDER: S-EPMC3521703 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
PLoS neglected tropical diseases 20121213 12
In this study we developed and evaluated a Brugia Hha I repeat loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Brugia genomic DNA. Amplification was detected using turbidity or fluorescence as readouts. Reactions generated a turbidity threshold value or a clear visual positive within 30 minutes using purified genomic DNA equivalent to one microfilaria. Similar results were obtained using DNA isolated from blood samples containing B. malayi microfilariae. Amplificat ...[more]