Dataset Information

Application of a high throughput method of biomarker discovery to improvement of the EarlyCDT(®)-Lung Test.

ABSTRACT:

Background

The National Lung Screening Trial showed that CT screening for lung cancer led to a 20% reduction in mortality. However, CT screening has a number of disadvantages including low specificity. A validated autoantibody assay is available commercially (EarlyCDT®-Lung) to aid in the early detection of lung cancer and risk stratification in patients with pulmonary nodules detected by CT. Recent advances in high throughput (HTP) cloning and expression methods have been developed into a discovery pipeline to identify biomarkers that detect autoantibodies. The aim of this study was to demonstrate the successful clinical application of this strategy to add to the EarlyCDT-Lung panel in order to improve its sensitivity and specificity (and hence positive predictive value, (PPV)).

Methods and findings

Serum from two matched independent cohorts of lung cancer patients were used (n?=?100 and n?=?165). Sixty nine proteins were initially screened on an abridged HTP version of the autoantibody ELISA using protein prepared on small scale by a HTP expression and purification screen. Promising leads were produced in shake flask culture and tested on the full assay. These results were analyzed in combination with those from the EarlyCDT-Lung panel in order to provide a set of re-optimized cut-offs. Five proteins that still displayed cancer/normal differentiation were tested for reproducibility and validation on a second batch of protein and a separate patient cohort. Addition of these proteins resulted in an improvement in the sensitivity and specificity of the test from 38% and 86% to 49% and 93% respectively (PPV improvement from 1 in 16 to 1 in 7).

Conclusion

This is a practical example of the value of investing resources to develop a HTP technology. Such technology may lead to improvement in the clinical utility of the EarlyCDT--Lung test, and so further aid the early detection of lung cancer.

SUBMITTER: Macdonald IK

PROVIDER: S-EPMC3521770 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Publications

Application of a high throughput method of biomarker discovery to improvement of the EarlyCDT(®)-Lung Test.

Macdonald Isabel K IK Murray Andrea A Healey Graham F GF Parsy-Kowalska Celine B CB Allen Jared J McElveen Jane J Robertson Chris C Sewell Herbert F HF Chapman Caroline J CJ Robertson John F R JF

PloS one 20121213 12

<h4>Background</h4>The National Lung Screening Trial showed that CT screening for lung cancer led to a 20% reduction in mortality. However, CT screening has a number of disadvantages including low specificity. A validated autoantibody assay is available commercially (EarlyCDT®-Lung) to aid in the early detection of lung cancer and risk stratification in patients with pulmonary nodules detected by CT. Recent advances in high throughput (HTP) cloning and expression methods have been developed into ...[more]

PMID: 23272083

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Dataset Information

Application of a high throughput method of biomarker discovery to improvement of the EarlyCDT(®)-Lung Test.

Background

Methods and findings

Conclusion

Publications

Application of a high throughput method of biomarker discovery to improvement of the EarlyCDT(®)-Lung Test.

Similar Datasets

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