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Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.

ABSTRACT: The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

SUBMITTER: Liu B 

PROVIDER: S-EPMC3522111 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.

Liu Bing B   Garnett James A JA   Lee Wei-chao WC   Lin Jing J   Salgado Paula P   Taylor Jonathan J   Xu Yingqi Y   Lambert Sebastian S   Cota Ernesto E   Matthews Steve S  

Biochemical and biophysical research communications 20120404 2

The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD. ...[more]

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