Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.
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ABSTRACT:
SUBMITTER: Liu B
PROVIDER: S-EPMC3522111 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Publications
Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.
Liu Bing B Garnett James A JA Lee Wei-chao WC Lin Jing J Salgado Paula P Taylor Jonathan J Xu Yingqi Y Lambert Sebastian S Cota Ernesto E Matthews Steve S
Biochemical and biophysical research communications 20120404 2
The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD. ...[more]
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