Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of deuterium oxide on Arabidopsis gene expression

ABSTRACT: 2H2O has a long history as a protein or amino acid labeling techinique, and such labeling systems have proven effective for many different types of studies. A disadvantage of a 2H2O labeling system is that plant growth is inhibited as the percentage of deuterium in the medium increases. However the molecular effects of 2H2O on plant growth has not previoulsly been investigated. To assist with the experimental design of proteomic 2H2O labeling studies, we used microarrays to determine the global effects of 2H2O on gene expression and to indentify genes which genes are effected by 2H2O treatment.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE18153 | GEO | 2009/09/18

SECONDARY ACCESSION(S): PRJNA119489

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of Arabidopsis after deuterium oxide treatment

Project description:2H2O has a long history as a protein or amino acid labeling techinique, and such labeling systems have proven effective for many different types of studies. A disadvantage of a 2H2O labeling system is that plant growth is inhibited as the percentage of deuterium in the medium increases. However the molecular effects of 2H2O on plant growth has not previoulsly been investigated. To assist with the experimental design of proteomic 2H2O labeling studies, we used microarrays to determine the global effects of 2H2O on gene expression and to indentify genes which genes are effected by 2H2O treatment. Experiment Overall Design: RNA was prepared from Arabidopsis seedlings grown for seven days on either H2O control media or 30% 2H2O to examine the long-term effects of 2H2O treatment,and from seedlings shifted from H2O media to either 2H2O or H2O for 4 h to examine short-term effects on gene expression, and seedlings shifted from 2H2O to H2O for 4 h to examine recovery effects on gene expression.

2009-09-25 | E-GEOD-18153 | biostudies-arrayexpress

Effect of metal ion on itaconic acid fermentability for Aspergillus terreus

Project description:Zn (Ⅱ) and Fe (Ⅱ) are the essential metal elements for the growth of microorganisms. It illustrated that more itaconic acid was achieved for 0.25 g/L ZnSO4·2H2O and 0.40 g/L FeSO4·2H2O than the control with 0.15 g/L ZnSO4·2H2O and 0.16 g/L FeSO4·2H2O after single factor assays for Aspergillus terreus.We furhter carried out transcriptome assays to uncover molecular mechanism of the enhanced itaconic acid fermentability for A. terreus with metal ion.Therefore, our study would provide a reference metal ion concentration ratio for itaconic and other biochemicals production.

2024-12-17 | GSE279212 | GEO

Project description:studies of microbial diversity on soil effected by antibiotic

| PRJNA874888 | ENA

Project description:High-deuterium sequencing

| PRJNA1125856 | ENA

GNPS Comparative metabolomics of diverse fungi crmA mutants

Project description:Comparative metabolomics of crmA mutants for Aspergillus fumigatus, Penicillium commune, P. camemberti, P. expansum, and Cochliobolus heterostrophus C5, co-culture of A. fumigatus and P. expansum, and deuterium labeling extractions

2022-04-05 | MSV000089206 | GNPS

Effect of D2O on S. pombe gene expression

Project description:RNA sequencing of total RNA purified from wild type S. pombe cells after 0, 5 and 24 hours culture in YES containing 100% D2O (deuterium oxide).

2020-01-01 | GSE119785 | GEO

Pseudomonas aeroginosa resistant against Ciprofloxacin

Project description:To indentify protein networks and proteins involved in Ciprofloxacin resistance developed by Pseudomonas aeruginosa

2016-12-12 | PXD004560 | Pride

Oleic acid-dependent modulation of Nitric Oxide Associated 1 protein levels regulate nitric oxide mediate signaling in plant defense.

Project description:Oleic acid-dependent modulation of Nitric Oxide Associated 1 protein levels regulate nitric oxide- mediate signaling in plant defense. Nitric Oxide, Oleic acid, Salicylic acid, Arabidopsis, Defense

2012-08-01 | GSE36797 | GEO

The transcriptome analysis of WUS targets

Project description:WUS is a key transcription factor and plays important role in plant stem cell maintenance. WUS expresses in organizing center and its protein moves upward to stem cells to activate CLV3 expressions. Only a few target genes of WUS were reported which involved in stem cell maintenance. To indentify new targets of WUS, we performed RNA high throughput sequencing after inducing WUS activity with DEX (dexamethasone) and CHX (Cycloheximide).

2021-02-22 | GSE128778 | GEO

Copper oxide nanomaterial regulation and fate in Cucurbita pepo

Project description:Nanomaterials (NMs) are on the nanoscale level range ca. 1-100 nm and due to the peculiar physico-chemical properties are widely used in different areas. The present study aimed to characterize the responses of zucchini (Cucurbita pepo L.) treated with copper oxide (CuO) NPs from seed germination to the flowering stage. CuO NPs treatment did not negatively impact plant morphology and growth as well as the pollen formation and viability. Physiological analyses were followed by a complete RNAseq-based transcriptomic analysis in the different plant tissues. Mitochondrial and chloroplast functions emerged as a critical component in plant response. Evidence of CuO NPs internalization and biotransformation was a driving force to measure the impacts at both physiological and molecular levels.

2021-07-25 | GSE173716 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data