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Effective flow cytometric phenotyping of cells using minimal amounts of antibody.

ABSTRACT: Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens.

SUBMITTER: Sharma D

PROVIDER: S-EPMC3523330 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Effective flow cytometric phenotyping of cells using minimal amounts of antibody.

Sharma Deepak D Eichelberg Mark R MR Haag Jill D JD Meilahn Amanda L AL Muelbl Matthew J MJ Schell Kathy K Smits Bart M G BM Gould Michael N MN

BioTechniques 20120701 1

Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens. ...[more]

PMID: 22780320

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Effective flow cytometric phenotyping of cells using minimal amounts of antibody.

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Effective flow cytometric phenotyping of cells using minimal amounts of antibody.

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