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Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells.

ABSTRACT: We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2 kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β(3) integrin cluster and induced specific myogenic differentiation at both transcription and translation levels with expression of myosin heavy chain and α-sarcomeric actin. This was initiated with elongation of FA complexes that triggered the RhoA downstream signaling toward a myogenic lineage commitment. This study also illustrates how one could partially control myogenic differentiation outcomes of similar-shaped hMSCs by modulating FA morphology and distribution. This technology increases our toolkit choice for controlled differentiation in muscle engineering.

SUBMITTER: Yu H 

PROVIDER: S-EPMC3528092 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Insights into the role of focal adhesion modulation in myogenic differentiation of human mesenchymal stem cells.

Yu Haiyang H   Lui Yuan Siang YS   Xiong Sijing S   Leong Wen Shing WS   Wen Feng F   Nurkahfianto Himawan H   Rana Sravendra S   Leong David Tai DT   Ng Kee Woei KW   Tan Lay Poh LP  

Stem cells and development 20120816 1

We report the establishment of a novel platform to induce myogenic differentiation of human mesenchymal stem cells (hMSCs) via focal adhesion (FA) modulation, giving insights into the role of FA on stem cell differentiation. Micropatterning of collagen type I on a polyacrylamide gel with a stiffness of 10.2 kPa efficiently modulated elongated FA. This elongated FA profile preferentially recruited the β(3) integrin cluster and induced specific myogenic differentiation at both transcription and tr  ...[more]

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