Ontology highlight
ABSTRACT:
SUBMITTER: Son S
PROVIDER: S-EPMC4626238 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Son Seoyoung S Liang Mao-Shih MS Lei Pedro P Xue Xiaozheng X Furlani Edward P EP Andreadis Stelios T ST
Bioconjugate chemistry 20150310 7
We used magnetofection (MF) to achieve high transfection efficiency into human mesenchymal stem cells (MSCs). A custom-made magnet array, matching well-to-well to a 24-well plate, was generated and characterized. Theoretical predictions of magnetic force distribution within each well demonstrated that there was no magnetic field interference among magnets in adjacent wells. An optimized protocol for efficient gene delivery to human hair follicle derived MSCs (hHF-MSCs) was established using an e ...[more]