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Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses.

ABSTRACT: The ability to visualize myelin is important in the diagnosis of demyelinating disorders and the detection of myelin-containing nerves during surgery. The development of myelin-selective imaging agents requires that a defined target for these agents be identified and that a robust assay against the target be developed to allow for assessment of structure-activity relationships. We describe an immunohistochemical analysis and a fluorescence polarization binding assay using purified myelin basic protein (MBP) that provides quantitative evidence that MBP is the molecular binding partner of previously described myelin-selective fluorescent dyes such as BMB, GE3082, and GE3111.

SUBMITTER: Bajaj A

PROVIDER: S-EPMC3534315 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Identification of the protein target of myelin-binding ligands by immunohistochemistry and biochemical analyses.

Bajaj Anshika A LaPlante Nicole E NE Cotero Victoria E VE Fish Kenneth M KM Bjerke Roger M RM Siclovan Tiberiu T Tan Hehir Cristina A CA

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 20121023 1

The ability to visualize myelin is important in the diagnosis of demyelinating disorders and the detection of myelin-containing nerves during surgery. The development of myelin-selective imaging agents requires that a defined target for these agents be identified and that a robust assay against the target be developed to allow for assessment of structure-activity relationships. We describe an immunohistochemical analysis and a fluorescence polarization binding assay using purified myelin basic p ...[more]

PMID: 23092790

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