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Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells.


ABSTRACT: Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs than do Matrigel and intact laminin isoforms. Furthermore, LM-E8s sustain long-term self-renewal of hESCs and hiPSCs in defined xeno-free media with dissociated cell passaging. We successfully maintained three hESC and two hiPSC lines on LM-E8s in three defined media for 10 passages. hESCs maintained high level expression of pluripotency markers, had a normal karyotype after 30 passages and could differentiate into all three germ layers. This culture system allows robust proliferation of hESCs and hiPSCs for therapeutic applications.

SUBMITTER: Miyazaki T 

PROVIDER: S-EPMC3535336 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells.

Miyazaki Takamichi T   Futaki Sugiko S   Suemori Hirofumi H   Taniguchi Yukimasa Y   Yamada Masashi M   Kawasaki Miwa M   Hayashi Maria M   Kumagai Hideaki H   Nakatsuji Norio N   Sekiguchi Kiyotoshi K   Kawase Eihachiro E  

Nature communications 20120101


Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential to provide an infinite source of tissues for regenerative medicine. Although defined xeno-free media have been developed, culture conditions for reliable propagation of hESCs still require considerable improvement. Here we show that recombinant E8 fragments of laminin isoforms (LM-E8s), which are the minimum fragments conferring integrin-binding activity, promote greater adhesion of hESCs and hiPSCs  ...[more]

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