Ontology highlight
ABSTRACT:
SUBMITTER: Curti E
PROVIDER: S-EPMC3536392 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Curti Elena E Jaeger Joachim J
Journal of virology 20121017 1
Residues Arg283, Arg285, and Ile287 are highly conserved amino acids in bovine viral diarrhea virus RNA polymerase (BVDV RdRp) and RdRps from related positive-strand RNA viruses. This motif is an important part of the binding pocket for the nascent RNA base pair during initiation and elongation. We found that replacement of the arginines with alanines or more conserved lysines or replacement of isoleucine with alanine or valine alters the ability of the mutant RdRps to incorporate ribonucleotide ...[more]