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Imaging the cell surface and its organization down to the level of single molecules.

ABSTRACT: Determining the organization of key molecules on the surface of live cells in two dimensions and how this changes during biological processes, such as signalling, is a major challenge in cell biology and requires methods with nanoscale spatial resolution and high temporal resolution. Here, we review biophysical tools, based on scanning ion conductance microscopy and single-molecule fluorescence and the combination of both of these methods, which have recently been developed to address these issues. We then give examples of how these methods have been be applied to provide new insights into cell membrane organization and function, and discuss some of the issues that will need to be addressed to further exploit these methods in the future.

SUBMITTER: Klenerman D

PROVIDER: S-EPMC3538430 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Imaging the cell surface and its organization down to the level of single molecules.

Klenerman David D Shevchuk Andrew A Novak Pavel P Korchev Yuri E YE Davis Simon J SJ

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20121224 1611

Determining the organization of key molecules on the surface of live cells in two dimensions and how this changes during biological processes, such as signalling, is a major challenge in cell biology and requires methods with nanoscale spatial resolution and high temporal resolution. Here, we review biophysical tools, based on scanning ion conductance microscopy and single-molecule fluorescence and the combination of both of these methods, which have recently been developed to address these issu ...[more]

PMID: 23267181

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