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Genetically encoded pH sensor for tracking surface proteins through endocytosis.

ABSTRACT: Traffic cam: a tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of ?(2)-adrenergic receptors. It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).

SUBMITTER: Grover A

PROVIDER: S-EPMC3538816 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Genetically encoded pH sensor for tracking surface proteins through endocytosis.

Grover Anmol A Schmidt Brigitte F BF Salter Russell D RD Watkins Simon C SC Waggoner Alan S AS Bruchez Marcel P MP

Angewandte Chemie (International ed. in English) 20120329 20

Traffic cam: a tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β(2)-adrenergic receptors. It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown). ...[more]

PMID: 22461279

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