Design and testing of a synthetic biology framework for genetic engineering of Corynebacterium glutamicum.

ABSTRACT:

Background

Synthetic biology approaches can make a significant contribution to the advance of metabolic engineering by reducing the development time of recombinant organisms. However, most of synthetic biology tools have been developed for Escherichia coli. Here we provide a platform for rapid engineering of C. glutamicum, a microorganism of great industrial interest. This bacteria, used for decades for the fermentative production of amino acids, has recently been developed as a host for the production of several economically important compounds including metabolites and recombinant proteins because of its higher capacity of secretion compared to traditional bacterial hosts like E. coli. Thus, the development of modern molecular platforms may significantly contribute to establish C

SUBMITTER: Ravasi P 

PROVIDER: S-EPMC3539996 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature