Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.
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ABSTRACT:
SUBMITTER: Chiba J
PROVIDER: S-EPMC3541039 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G.
Chiba Junya J Kouno Takahide T Aoki Shun S Sato Hitoshi H Zhang JingYing J Matsuo Hiroshi H Inouye Masahiko M
Chemical communications (Cambridge, England) 20121112 99
APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications. ...[more]
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