Ontology highlight
ABSTRACT:
SUBMITTER: Chi SW
PROVIDER: S-EPMC3541676 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Chi Sung Wook SW Hannon Gregory J GJ Darnell Robert B RB
Nature structural & molecular biology 20120212 3
MicroRNAs (miRNAs) regulate mRNA targets through perfect pairing with their seed region (positions 2-7). Recently, a precise genome-wide map of miRNA interaction sites in mouse brain was generated by high-throughput sequencing and analysis of clusters of ~50-nucleotide mRNA tags cross-linked to Argonaute (Ago HITS-CLIP). By analyzing Ago HITS-CLIP 'orphan clusters'-Ago binding regions from HITS-CLIP that cannot be explained by canonical seed matches-we have now identified an alternative binding ...[more]