Ontology highlight
ABSTRACT:
SUBMITTER: Kim J
PROVIDER: S-EPMC3541820 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Kim Jieun J Rubin Nicole N Huang Ying Y Tuan Tai-Lan TL Lien Ching-Ling CL
Nature protocols 20120119 2
We describe here a protocol for culturing epicardial cells from adult zebrafish hearts, which have a unique regenerative capacity after injury. Briefly, zebrafish hearts first undergo ventricular amputation or sham operation. Next, the hearts are excised and explanted onto fibrin gels prepared in advance in a multiwell tissue culture plate. The procedure allows the epicardial cells to outgrow from the ventricle onto a fibrin matrix in vitro. This protocol differs from those used in other organis ...[more]