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Tcf21+ epicardial cells adopt non-myocardial fates during zebrafish heart development and regeneration.

ABSTRACT: Recent lineage-tracing studies have produced conflicting results about whether the epicardium is a source of cardiac muscle cells during heart development. Here, we examined the developmental potential of epicardial tissue in zebrafish during both embryonic development and injury-induced heart regeneration. We found that upstream sequences of the transcription factor gene tcf21 activated robust, epicardium-specific expression throughout development and regeneration. Cre recombinase-based, genetic fate-mapping of larval or adult tcf21(+) cells revealed contributions to perivascular cells, but not cardiomyocytes, during each form of cardiogenesis. Our findings indicate that natural epicardial fates are limited to non-myocardial cell types in zebrafish.

SUBMITTER: Kikuchi K

PROVIDER: S-EPMC3119303 | biostudies-other | 2011 Jul

REPOSITORIES: biostudies-other

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tcf21+ epicardial cells adopt non-myocardial fates during zebrafish heart development and regeneration.

Kikuchi Kazu K Gupta Vikas V Wang Jinhu J Holdway Jennifer E JE Wills Airon A AA Fang Yi Y Poss Kenneth D KD

Development (Cambridge, England) 20110608 14

Recent lineage-tracing studies have produced conflicting results about whether the epicardium is a source of cardiac muscle cells during heart development. Here, we examined the developmental potential of epicardial tissue in zebrafish during both embryonic development and injury-induced heart regeneration. We found that upstream sequences of the transcription factor gene tcf21 activated robust, epicardium-specific expression throughout development and regeneration. Cre recombinase-based, geneti ...[more]

PMID: 21653610

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