Ontology highlight
ABSTRACT:
SUBMITTER: Boonsri P
PROVIDER: S-EPMC3544975 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Biochemical and biophysical research communications 20121110 1
Phosphomevalonate kinase (PMK) phosphorylates mevalonate-5-phosphate (M5P) in the mevalonate pathway, which is the sole source of isoprenoids and steroids in humans. We have identified new PMK inhibitors with virtual screening, using autodock. Promising hits were verified and their affinity measured using NMR-based (1)H-(15)N heteronuclear single quantum coherence (HSQC) chemical shift perturbation and fluorescence titrations. Chemical shift changes were monitored, plotted, and fitted to obtain ...[more]