Molecular identification, cloning and characterization of transmitted/founder HIV-1 subtype A, D and A/D infectious molecular clones.
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ABSTRACT:
SUBMITTER: Baalwa J
PROVIDER: S-EPMC3545109 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Publications
Molecular identification, cloning and characterization of transmitted/founder HIV-1 subtype A, D and A/D infectious molecular clones.
Baalwa Joshua J Wang Shuyi S Parrish Nicholas F NF Decker Julie M JM Keele Brandon F BF Learn Gerald H GH Yue Ling L Ruzagira Eugene E Ssemwanga Deogratius D Kamali Anatoli A Amornkul Pauli N PN Price Matt A MA Kappes John C JC Karita Etienne E Kaleebu Pontiano P Sanders Eduard E Gilmour Jill J Allen Susan S Hunter Eric E Montefiori David C DC Haynes Barton F BF Cormier Emmanuel E Hahn Beatrice H BH Shaw George M GM
Virology 20121101 1
We report the molecular identification, cloning and initial biological characterization of 12 full-length HIV-1 subtype A, D and A/D recombinant transmitted/founder (T/F) genomes. T/F genomes contained intact canonical open reading frames and all T/F viruses were replication competent in primary human T-cells, although subtype D virus replication was more efficient (p<0.05). All 12 viruses utilized CCR5 but not CXCR4 as a co-receptor for entry and exhibited a neutralization profile typical of ti ...[more]
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