Ontology highlight
ABSTRACT:
SUBMITTER: Sample V
PROVIDER: S-EPMC3549020 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Sample Vedangi V Ni Qiang Q Mehta Sohum S Inoue Takanari T Zhang Jin J
ACS chemical biology 20121026 1
Molecular probes designed to monitor or perturb signaling events in living cells rely on engineered molecular switches. Here, we show that a kinase-inducible bimolecular switch comprising a kinase-specific substrate and a phosphoamino acid binding domain can be used for acute regulation of cellular events. As a proof of concept, we employed a Protein Kinase A (PKA)-dependent switch and coupled it to a lipid phosphatase to manipulate the level of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2) ...[more]