Ontology highlight
ABSTRACT:
SUBMITTER: Delker RK
PROVIDER: S-EPMC3549133 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Delker Rebecca K RK Zhou Yanjiao Y Strikoudis Alexandros A Stebbins C Erec CE Papavasiliou F Nina FN
Proceedings of the National Academy of Sciences of the United States of America 20121231 3
Protein-protein interactions are typically identified by either biochemical purification coupled to mass spectrometry or genetic approaches exemplified by the yeast two-hybrid assay; however, neither assay works well for the identification of cofactors for poorly soluble proteins. Solubility of a poorly soluble protein is thought to increase upon cofactor binding, possibly by masking otherwise exposed hydrophobic domains. We have exploited this notion to develop a high-throughput genetic screen ...[more]