Ontology highlight
ABSTRACT:
SUBMITTER: Sherpa AR
PROVIDER: S-EPMC3550814 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Sherpa A R AR Hallan V V Zaidi A A AA
Indian journal of virology : an official organ of Indian Virological Society 20120427 1
Polyclonal rabbit antisera were produced using coat protein of Cymbidium mosaic virus (CymMV) Indian isolate expressed in E. coli as GST fusion. The expressed protein was purified by GST-fusion protein purification kit for use as an immunogen in rabbits. Antisera prepared in this manner reacted in double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) with extract from CymMV-infected tissue. The results indicate that polyclonal antisera prepared from expressed CymMV coat proteins ...[more]