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Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter.

ABSTRACT: In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.

SUBMITTER: Carter L

PROVIDER: S-EPMC3553758 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter.

Carter L L Lindsey L A LA Grim C J CJ Sathyamoorthy V V Jarvis K G KG Gopinath G G Lee C C Sadowski J A JA Trach L L Pava-Ripoll M M McCardell B A BA Tall B D BD Hu L L

Applied and environmental microbiology 20121109 2

In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations. ...[more]

PMID: 23144142

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