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Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.

ABSTRACT: We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host.

SUBMITTER: Damron FH

PROVIDER: S-EPMC3553786 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.

Damron F Heath FH McKenney Elizabeth S ES Schweizer Herbert P HP Goldberg Joanna B JB

Applied and environmental microbiology 20121102 2

We describe a mini-Tn7-based broad-host-range expression cassette for arabinose-inducible gene expression from the P(BAD) promoter. This delivery vector, pTJ1, can integrate a single copy of a gene into the chromosome of Gram-negative bacteria for diverse genetic applications, of which several are discussed, using Pseudomonas aeruginosa as the model host. ...[more]

PMID: 23124231

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