Unknown

Dataset Information

0

An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.

ABSTRACT: An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio.

SUBMITTER: Glassing A 

PROVIDER: S-EPMC4624500 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria.

Glassing Angela A   Lewis Thomas A TA  

Journal of microbiological methods 20150901

An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio. ...[more]

Similar Datasets

Unknown Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.
| S-EPMC3697578 | biostudies-literature
Unknown Construction of a broad-host-range Tn7-based vector for single-copy P(BAD)-controlled gene expression in gram-negative bacteria.
| S-EPMC3553786 | biostudies-literature
Unknown Reconstructing promoter activity from Lux bioluminescent reporters.
| S-EPMC5619816 | biostudies-literature
Unknown Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids.
| S-EPMC168554 | biostudies-other
Unknown Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4.
| S-EPMC176555 | biostudies-other
Unknown Identification of surprisingly diverse type IV pili, across a broad range of gram-positive bacteria.
| S-EPMC3244431 | biostudies-literature
Unknown Improved broad-spectrum antibiotics against Gram-negative pathogens <i>via</i> darobactin biosynthetic pathway engineering.
| S-EPMC8442675 | biostudies-literature
Unknown LTR12 promoter activation in a broad range of human tumor cells by HDAC inhibition.
| S-EPMC5085097 | biostudies-literature
Unknown Development of a thermally regulated broad-spectrum promoter system for use in pathogenic gram-positive species.
| S-EPMC161472 | biostudies-literature
Unknown Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria.
| S-EPMC5336243 | biostudies-literature