Dataset Information

Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil.

ABSTRACT: Microarray analyses revealed that the expression of genes for secondary metabolism together with that of primary metabolic genes was induced by chitin in autoclaved soil cultures of Streptomyces coelicolor A3(2). The data also indicated that DasR was involved in the regulation of gene expression for chitin catabolism, secondary metabolism, and stress responses.

SUBMITTER: Nazari B

PROVIDER: S-EPMC3553787 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil.

Nazari Behnam B Kobayashi Michihiko M Saito Akihiro A Hassaninasab Azam A Miyashita Kiyotaka K Fujii Takeshi T

Applied and environmental microbiology 20121102 2

Microarray analyses revealed that the expression of genes for secondary metabolism together with that of primary metabolic genes was induced by chitin in autoclaved soil cultures of Streptomyces coelicolor A3(2). The data also indicated that DasR was involved in the regulation of gene expression for chitin catabolism, secondary metabolism, and stress responses. ...[more]

PMID: 23124229

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Project description:BackgroundSystems biology approaches to study metabolic switching in Streptomyces coelicolor A3(2) depend on cultivation conditions ensuring high reproducibility and distinct phases of culture growth and secondary metabolite production. In addition, biomass concentrations must be sufficiently high to allow for extensive time-series sampling before occurrence of a given nutrient depletion for transition triggering. The present study describes for the first time the development of a dedicated optimized submerged batch fermentation strategy as the basis for highly time-resolved systems biology studies of metabolic switching in S. coelicolor A3(2).ResultsBy a step-wise approach, cultivation conditions and two fully defined cultivation media were developed and evaluated using strain M145 of S. coelicolor A3(2), providing a high degree of cultivation reproducibility and enabling reliable studies of the effect of phosphate depletion and L-glutamate depletion on the metabolic transition to antibiotic production phase. Interestingly, both of the two carbon sources provided, D-glucose and L-glutamate, were found to be necessary in order to maintain high growth rates and prevent secondary metabolite production before nutrient depletion. Comparative analysis of batch cultivations with (i) both L-glutamate and D-glucose in excess, (ii) L-glutamate depletion and D-glucose in excess, (iii) L-glutamate as the sole source of carbon and (iv) D-glucose as the sole source of carbon, reveal a complex interplay of the two carbon sources in the bacterium's central carbon metabolism.ConclusionsThe present study presents for the first time a dedicated cultivation strategy fulfilling the requirements for systems biology studies of metabolic switching in S. coelicolor A3(2). Key results from labelling and cultivation experiments on either or both of the two carbon sources provided indicate that in the presence of D-glucose, L-glutamate was the preferred carbon source, while D-glucose alone appeared incapable of maintaining culture growth, likely due to a metabolic bottleneck at the oxidation of pyruvate to acetyl-CoA.

Dataset Information

Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil.

Publications

Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil.

Similar Datasets

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