Ontology highlight
ABSTRACT:
SUBMITTER: Delacote F
PROVIDER: S-EPMC3554739 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
PloS one 20130124 1
Targeting DNA double-strand breaks is a powerful strategy for gene inactivation applications. Without the use of a repair plasmid, targeted mutagenesis can be achieved through Non-Homologous End joining (NHEJ) pathways. However, many of the DNA breaks produced by engineered nucleases may be subject to precise re-ligation without loss of genetic information and thus are likely to be unproductive. In this study, we combined engineered endonucleases and DNA-end processing enzymes to increase the ef ...[more]