Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.
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ABSTRACT:
SUBMITTER: Certo MT
PROVIDER: S-EPMC3602999 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Publications
Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.
Certo Michael T MT Gwiazda Kamila S KS Kuhar Ryan R Sather Blythe B Curinga Gabrielle G Mandt Tyler T Brault Michelle M Lambert Abigail R AR Baxter Sarah K SK Jacoby Kyle K Ryu Byoung Y BY Kiem Hans-Peter HP Gouble Agnes A Paques Frederic F Rawlings David J DJ Scharenberg Andrew M AM
Nature methods 20120902 10
Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements ...[more]
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