High-throughput interrogation of ligand binding mode using a fluorescence-based assay.
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ABSTRACT:
SUBMITTER: Sledz P
PROVIDER: S-EPMC3556687 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
High-throughput interrogation of ligand binding mode using a fluorescence-based assay.
Śledź Paweł P Lang Steffen S Stubbs Christopher J CJ Abell Chris C
Angewandte Chemie (International ed. in English) 20120622 31
Probing the pocket: A high-throughput fluorescence-based thermal shift (FTS) assay utilized different forms of a protein (in gray) to establish the binding mode of a ligand (see picture). The assay serves in the rapid evaluation of structure-activity binding-mode relationships for a series of ligands of Plk1, an important target of anticancer therapy. ...[more]
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