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Cytokinins can act as suppressors of nitric oxide in Arabidopsis.


ABSTRACT: Maintaining nitric oxide (NO) homeostasis is essential for normal plant physiological processes. However, very little is known about the mechanisms of NO modulation in plants. Here, we report a unique mechanism for the catabolism of NO based on the reaction with the plant hormone cytokinin. We screened for NO-insensitive mutants in Arabidopsis and isolated two allelic lines, cnu1-1 and 1-2 (continuous NO-unstressed 1), that were identified as the previously reported altered meristem program 1 (amp1) and as having elevated levels of cytokinins. A double mutant of cnu1-2 and nitric oxide overexpression 1 (nox1) reduced the severity of the phenotypes ascribed to excess NO levels as did treating the nox1 line with trans-zeatin, the predominant form of cytokinin in Arabidopsis. We further showed that peroxinitrite, an active NO derivative, can react with zeatin in vitro, which together with the results in vivo suggests that cytokinins suppress the action of NO most likely through direct interaction between them, leading to the reduction of endogenous NO levels. These results provide insights into NO signaling and regulation of its bioactivity in plants.

SUBMITTER: Liu WZ 

PROVIDER: S-EPMC3557067 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Cytokinins can act as suppressors of nitric oxide in Arabidopsis.

Liu Wei-Zhong WZ   Kong Dong-Dong DD   Gu Xue-Xin XX   Gao Hong-Bo HB   Wang Jin-Zheng JZ   Xia Min M   Gao Qian Q   Tian Li-Li LL   Xu Zhang-Hong ZH   Bao Fang F   Hu Yong Y   Ye Neng-Sheng NS   Pei Zhen-Ming ZM   He Yi-Kun YK  

Proceedings of the National Academy of Sciences of the United States of America 20130114 4


Maintaining nitric oxide (NO) homeostasis is essential for normal plant physiological processes. However, very little is known about the mechanisms of NO modulation in plants. Here, we report a unique mechanism for the catabolism of NO based on the reaction with the plant hormone cytokinin. We screened for NO-insensitive mutants in Arabidopsis and isolated two allelic lines, cnu1-1 and 1-2 (continuous NO-unstressed 1), that were identified as the previously reported altered meristem program 1 (a  ...[more]

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