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Post-oral glucose stimulation of intake and conditioned flavor preference in C57BL/6J mice: a concentration-response study.

ABSTRACT: In a recent study, intragastric (IG) self-infusion of 16% glucose stimulated 1-h intake and conditioned a preference for a flavored saccharin solution in C57BL/6J mice (Zukerman et al., 2011). Experiment 1 of the present study presents a concentration-response analysis of IG glucose-induced intake stimulation monitored by recording licking response every min of the 1h/day sessions. Separate groups of food-restricted mice consumed a flavored saccharin solution (the CS-) paired with IG self-infusions of water (Test 0) followed by a different flavored solution (the CS+) paired with IG self-infusions of 2, 4, 8, 16, or 32% glucose (Tests 1-3). Following additional CS- and CS+ training sessions, a two-bottle CS+ vs. CS- choice test was conducted without infusions. Self-infusions of 8%, 16% or 32% glucose stimulated CS+ licking within 12 min of the first test session and even earlier in subsequent test sessions, and also conditioned significant CS+ preferences in the two-bottle test. The stimulation of early licking and CS+ preference increased as a function of glucose concentration. The amount of glucose solute self-infused increased with sugar concentration as did post-infusion blood glucose levels. The 2% glucose infusion did not stimulate CS+ intake and the 2% and 4% infusions failed to produce a CS+ preference in the 1-h test. Experiment 2 revealed that intraperitoneal self-infusions of 8% glucose, unlike IG glucose self-infusions, failed to stimulate CS+ licking or preference despite producing maximal increases in blood glucose levels. Taken together, these and other findings suggest that glucose rapidly produces concentration-dependent intestinal signals that stimulate intake and condition flavor preferences while post-oral satiation signals limit total amounts consumed.

SUBMITTER: Zukerman S

PROVIDER: S-EPMC3557835 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Post-oral glucose stimulation of intake and conditioned flavor preference in C57BL/6J mice: a concentration-response study.

Zukerman Steven S Ackroff Karen K Sclafani Anthony A

Physiology & behavior 20121128

In a recent study, intragastric (IG) self-infusion of 16% glucose stimulated 1-h intake and conditioned a preference for a flavored saccharin solution in C57BL/6J mice (Zukerman et al., 2011). Experiment 1 of the present study presents a concentration-response analysis of IG glucose-induced intake stimulation monitored by recording licking response every min of the 1h/day sessions. Separate groups of food-restricted mice consumed a flavored saccharin solution (the CS-) paired with IG self-infusi ...[more]

PMID: 23200639

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Project description:A high caloric diet, in conjunction with low levels of physical activity, promotes obesity. Many studies are available regarding the relation between dietary saturated fats and the etiology of obesity, but most focus on liver, muscle and white adipose tissue. Furthermore, the majority of transcriptomic studies seek to identify linear effects of an external stimulus on gene expression, although such an assumption does not necessarily hold. Our work assesses the dose-dependent effects of dietary fat intake on differential gene expression in the proximal, middle and distal sections of the small intestine in C57BL/6J mice. Gene expression is analyzed in terms of either linear or nonlinear responses to fat intake.The highest number of differentially expressed genes was observed in the middle section. In all intestine sections, most of the identified processes exhibited a linear response to increasing fat intake. The relative importance of logarithmic and exponential responses was higher in the proximal and distal sections, respectively. Functional enrichment analysis highlighted a constantly linear regulation of acute-phase response along the whole small intestine, with up-regulation of Serpina1b. The study of gene expression showed that exponential down-regulation of cholesterol transport in the middle section is coupled with logarithmic up-regulation of cholesterol homeostasis. A shift from linear to exponential response was observed in genes involved in the negative regulation of caspase activity, from middle to distal section (e.g., Birc5, up-regulated).The transcriptomic signature associated with inflammatory processes preserved a linear response in the whole small intestine (e.g., up-regulation of Serpina1b). Processes related to cholesterol homeostasis were particularly active in the middle small intestine and only the highest fat intake down-regulated cholesterol transport and efflux (with a key role played by the down-regulation of ATP binding cassette transporters). Characterization of nonlinear patterns of gene expression triggered by different levels of dietary fat is an absolute novelty in intestinal studies. This approach helps identifying which processes are overloaded (i.e., positive, logarithmic regulation) or arrested (i.e., negative, exponential regulation) in response to excessive fat intake, and can shed light on the relationships linking lipid intake to obesity and its associated molecular disturbances.

Dataset Information

Post-oral glucose stimulation of intake and conditioned flavor preference in C57BL/6J mice: a concentration-response study.

Publications

Post-oral glucose stimulation of intake and conditioned flavor preference in C57BL/6J mice: a concentration-response study.

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