Ontology highlight
ABSTRACT:
SUBMITTER: Moiani A
PROVIDER: S-EPMC3561312 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Moiani Arianna A Miccio Annarita A Rizzi Ermanno E Severgnini Marco M Pellin Danilo D Suerth Julia Debora JD Baum Christopher C De Bellis Gianluca G Mavilio Fulvio F
PloS one 20130131 1
Moloney murine leukemia virus (MLV)-derived gamma-retroviral vectors integrate preferentially near transcriptional regulatory regions in the human genome, and are associated with a significant risk of insertional gene deregulation. Self-inactivating (SIN) vectors carry a deletion of the U3 enhancer and promoter in the long terminal repeat (LTR), and show reduced genotoxicity in pre-clinical assays. We report a high-definition analysis of the integration preferences of a SIN MLV vector compared t ...[more]