Ontology highlight
ABSTRACT:
SUBMITTER: Yang F
PROVIDER: S-EPMC3562772 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Yang Fei F Nickols Nicholas G NG Li Benjamin C BC Marinov Georgi K GK Said Jonathan W JW Dervan Peter B PB
Proceedings of the National Academy of Sciences of the United States of America 20130114 5
Many cancer therapeutics target DNA and exert cytotoxicity through the induction of DNA damage and inhibition of transcription. We report that a DNA minor groove binding hairpin pyrrole-imidazole (Py-Im) polyamide interferes with RNA polymerase II (RNAP2) activity in cell culture. Polyamide treatment activates p53 signaling in LNCaP prostate cancer cells without detectable DNA damage. Genome-wide mapping of RNAP2 binding shows reduction of occupancy, preferentially at transcription start sites, ...[more]