Ontology highlight
ABSTRACT:
SUBMITTER: Catherman AD
PROVIDER: S-EPMC3861700 | biostudies-literature | 2013 Dec
REPOSITORIES: biostudies-literature
Catherman Adam D AD Durbin Kenneth R KR Ahlf Dorothy R DR Early Bryan P BP Fellers Ryan T RT Tran John C JC Thomas Paul M PM Kelleher Neil L NL
Molecular & cellular proteomics : MCP 20130910 12
Top-down proteomics is emerging as a viable method for the routine identification of hundreds to thousands of proteins. In this work we report the largest top-down study to date, with the identification of 1,220 proteins from the transformed human cell line H1299 at a false discovery rate of 1%. Multiple separation strategies were utilized, including the focused isolation of mitochondria, resulting in significantly improved proteome coverage relative to previous work. In all, 347 mitochondrial p ...[more]