Ontology highlight
ABSTRACT:
SUBMITTER: Dumitriu D
PROVIDER: S-EPMC3566769 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Dumitriu Dani D Rodriguez Alfredo A Morrison John H JH
Nature protocols 20110825 9
Morphological features such as size, shape and density of dendritic spines have been shown to reflect important synaptic functional attributes and potential for plasticity. Here we describe in detail a protocol for obtaining detailed morphometric analysis of spines using microinjection of fluorescent dyes, high-resolution confocal microscopy, deconvolution and image analysis with NeuronStudio. Recent technical advancements include better preservation of tissue, resulting in prolonged ability to ...[more]