Ontology highlight
ABSTRACT:
SUBMITTER: Sharei A
PROVIDER: S-EPMC3568376 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Sharei Armon A Zoldan Janet J Adamo Andrea A Sim Woo Young WY Cho Nahyun N Jackson Emily E Mao Shirley S Schneider Sabine S Han Min-Joon MJ Lytton-Jean Abigail A Basto Pamela A PA Jhunjhunwala Siddharth S Lee Jungmin J Heller Daniel A DA Kang Jeon Woong JW Hartoularos George C GC Kim Kwang-Soo KS Anderson Daniel G DG Langer Robert R Jensen Klavs F KF
Proceedings of the National Academy of Sciences of the United States of America 20130122 6
Intracellular delivery of macromolecules is a challenge in research and therapeutic applications. Existing vector-based and physical methods have limitations, including their reliance on exogenous materials or electrical fields, which can lead to toxicity or off-target effects. We describe a microfluidic approach to delivery in which cells are mechanically deformed as they pass through a constriction 30-80% smaller than the cell diameter. The resulting controlled application of compression and s ...[more]