Ontology highlight
ABSTRACT:
SUBMITTER: Sharei A
PROVIDER: S-EPMC3966949 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Sharei Armon A Poceviciute Roberta R Jackson Emily L EL Cho Nahyun N Mao Shirley S Hartoularos George C GC Jang Derek Y DY Jhunjhunwala Siddharth S Eyerman Alexandra A Schoettle Taylor T Langer Robert R Jensen Klavs F KF
Integrative biology : quantitative biosciences from nano to macro 20140205 4
Intracellular delivery of materials is a challenge in research and therapeutic applications. Physical methods of plasma membrane disruption have recently emerged as an approach to facilitate the delivery of a variety of macromolecules to a range of cell types. We use the microfluidic CellSqueeze delivery platform to examine the kinetics of plasma membrane recovery after disruption and its dependence on the calcium content of the surrounding buffer (recovery time ∼ 5 min without calcium vs. ∼ 30 ...[more]