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Rapid detection of Klebsiella pneumoniae from blood culture bottles by real-time PCR.

ABSTRACT: A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative blood cultures from double-blind experiments, thus demonstrating 100% specificity when compared to results of conventional biochemical characterization. The assay also showed 100% sensitivity, as it correctly identified all 142 positive-control blood cultures and 4 from double-blind trials.

SUBMITTER: Kurupati P

PROVIDER: S-EPMC356840 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature

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Rapid detection of Klebsiella pneumoniae from blood culture bottles by real-time PCR.

Kurupati Prathiba P Chow Carol C Kumarasinghe Gamini G Poh Chit Laa CL

Journal of clinical microbiology 20040301 3

A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative blood cultures from double-blind experiments, thus demonstrating 100% specificity when compared to res ...[more]

PMID: 15004113

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