Ontology highlight
ABSTRACT:
SUBMITTER: Quezada EM
PROVIDER: S-EPMC3568871 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Quezada Elizabeth M EM Kane Caroline M CM
The open biochemistry journal 20130131
The Hepatitis C Virus RNA dependent RNA polymerase, NS5B, is stimulated by the NS5A protein in vitro. To explore this stimulatory mechanism, we compared the activity of a mutant of NS5B containing a deletion of the β-loop region with that of the full length NS5B in response to NS5A. While the NS5A protein does stimulate full length NS5B, NS5A does not stimulate the NS5B deletion mutant during either replication initiation or elongation. This result suggests that the activation mechanism might in ...[more]