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Complete Genome Sequence of Sacbrood Virus Strain SBM2, Isolated from the Honeybee Apis cerana in Vietnam.

ABSTRACT: Here we report the complete genomic sequence of the SBM2 strain (VSBV-SBM2) of the sacbrood virus (SBV) that was isolated from the Asian honeybee (Apis cerana) in Northern Vietnam. The entire sequence excluding the 3' poly(A) tail is 8,834 nucleotides in length and contains a single large open reading frame (ORF) of 8,580 nucleotides (position 178 to 8757), encoding 2,859 amino acids. VSBV-SBM2 shared 90 to 93% nucleotide identity and 95 to 96% amino acid homology to six complete genomes of SBV currently available in GenBank (two from China, three from Korea, and one from the United Kingdom). A hypervariable domain (amino acid [aa] position 712 to 729) and a conserved motif (position 2124 to 2143) in the precursor polypeptide of all seven SBVs are also described.

SUBMITTER: Nguyen NT

PROVIDER: S-EPMC3569296 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Complete Genome Sequence of Sacbrood Virus Strain SBM2, Isolated from the Honeybee Apis cerana in Vietnam.

Nguyen Nga Thi Bich NT Le Thanh Hoa TH

Genome announcements 20130131 1

Here we report the complete genomic sequence of the SBM2 strain (VSBV-SBM2) of the sacbrood virus (SBV) that was isolated from the Asian honeybee (Apis cerana) in Northern Vietnam. The entire sequence excluding the 3' poly(A) tail is 8,834 nucleotides in length and contains a single large open reading frame (ORF) of 8,580 nucleotides (position 178 to 8757), encoding 2,859 amino acids. VSBV-SBM2 shared 90 to 93% nucleotide identity and 95 to 96% amino acid homology to six complete genomes of SBV ...[more]

PMID: 23405307

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Project description:We determined the complete genome sequence of a sacbrood virus (SBV) infecting Indian honey bee (Apis cerana indica) from Tamil Nadu, India named as AcSBV-IndTN1. The genome of AcSBV-IndTN1 comprised of 8740 nucleotides, encoding a single large ORF containing 2849 amino acids flanked by 5' and 3' untranslated regions. Results of phylogenetic tree analysis based on complete genomes of SBV isolates indicated that the virus isolates from India isolated from the Asiatic honey bee A. cerana (AcSBVs) formed a separate group along with six Vietnam isolates and three Chinese isolates. The AcSBV-IndTN1 isolate showed closer genetic relationship with other isolates from India. The second major group had both AcSBVs and AmSBVs (virus isolated from European honey bee, Apis mellifera SBV) of Korea, China and Vietnam. The third and a distantly related group had AmSBVs of Australia, UK, USA and Korea. The results obtained from phylogenetic analysis were further supported with evolutionary distance analysis. AcSBV-IndTN1 isolate open reading frame had 95-99% amino acid sequence similarity with other Indian isolates and 92-96% with AcSBVs and AmSBVs of other geographical locations. In addition, sequence difference count matrix ranged from 154 to 907 nt among all the SBV isolates. This suggests that the virus isolates have evolved significantly in different geographical locations but isolates on different hosts in a given location/country are closely related. The high similarity in the genome among the AcSBV and AmSBV isolates indicate possible cross-infections and recombination of SBV isolates in Asian continent where both the honey bee species are reared in close proximity. Gene flow between SBV population indicating that an infrequent gene flow occur between them. The pattern of molecular diversity in SBV population revealed that the occurrence of recent population expansion of SBV. To the best of our knowledge this is the first report of the complete nucleotide sequence of AcSBV from Tamil Nadu, India. This study provided an opportunity to establish the molecular evolution of SBV isolates and shall be useful in the development of diagnostics and effective disease control strategies.

Dataset Information

Complete Genome Sequence of Sacbrood Virus Strain SBM2, Isolated from the Honeybee Apis cerana in Vietnam.

Publications

Complete Genome Sequence of Sacbrood Virus Strain SBM2, Isolated from the Honeybee Apis cerana in Vietnam.

Similar Datasets

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