Ontology highlight
ABSTRACT:
SUBMITTER: Gupta S
PROVIDER: S-EPMC357029 | biostudies-literature | 2004 Feb
REPOSITORIES: biostudies-literature
Gupta Sunita S Schoer Rebecca A RA Egan James E JE Hannon Gregory J GJ Mittal Vivek V
Proceedings of the National Academy of Sciences of the United States of America 20040204 7
RNA interference is a powerful genetic approach for efficiently silencing target genes. The existing method of gene suppression by the constitutive expression of short hairpin RNAs (shRNAs) allows analysis of the consequences of stably silencing genes but limits the analysis of genes essential for cell survival, cell cycle regulation, and cell development. We have developed an inducible U6 promoter for synthesis of shRNAs in both human and murine cells. Cells containing stably integrated shRNA e ...[more]